Biochemical Engineering by James M. Lee

This book was published by Prentice Hall in 1992 and was converted to an ebook (PDF file) in 2001. It was last updated in 2010 and is widely taught in university classrooms today.


  • Many solved examples and chapter-end problems.
  • Solution examples using MathCad or Mathlab.
  • Clear explanations for enzyme and cell kinetics.
  • One pdf file with bookmarks for easy searching
  • Simple laboratory experiments.


“I have to say it’s a great book for a newbie in biochemical engineering. You made the main concept easier to understand than my 900-page textbook!”


1 Introduction:
Biotechnology and Biochemical Engineering
2 Enzyme Kinetics:
Kinetics, bioreactor design, inhibition, etc.
3 Immobilized Enzyme:
Immobilization techniques and effect of mass transfer resistance
4 Industrial Applications of Enzymes:
Carbohydrates, starch conversion, and cellulose conversion
5 Cell Cultivations:
Microbial, animal, and plant cell cultivations, cell growth measurement, and cell immobilization
6 Cell Kinetics and Fermenter Design:
Growth Cycle, Kinetics, batch and continuous stirred-tank fermenter, multiple fermenters in series, cell recycling, alternative fermenters, and structured kinetic models
7 Genetic Engineering:
DNA and RNA, cloning of genes, stability of recombinant cells, genetic engineering of plant cells
8 Sterilization:
Sterilization methods, thermal death kinetics, design criterion, batch and continuous sterilization, and air sterilization
9 Agitation and Aeration:
Basic mass-transfer concepts, measurement techniques, mass-transfer coefficient, interfacial area, gas hold-up, power consumption, oxygen absorption rate, volumetric mass transfer coefficient, scale-up, and shear sensitive mixing
10 Downstream Processing:
Filtration, centrifugation, cell rupture, extraction, adsorption, precipitation, chromatography, electrophoresis, membrane separation


Frequently, students are required to buy an expensive textbook for the sake of only a few chapters that their instructors wish to cover. This ebook covers the main topics of biochemical engineering that most instructors teach in their classes. 

Please read the FAQs if you have questions about adopting this ebook.

A solution manual will be provided upon request to instructors who have adopted this book as their main textbook.


James M. Lee, author of Biochemical Engineering
James M. Lee
The author, James M. Lee, was a professor of Chemical Engineering at Washington State
University. His main research areas were bioreactor design, cell and enzyme kinetics, plant
cell cultures, bioremediation, mixing, and mass transfer.


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